Biomolecular surface engineering of pancreatic islets with thrombomodulin

Surface re-engineering of pancreatic islets with recombinant azido-thrombomodulin.

Chemoselective conjugation of an azido-functionalized thrombomodulin to pancreatic islets was achieved by Staudinger ligation to a surface-bound bifunctional poly(ethylene glycol) linker. The presence of the tethered thrombomodulin resulted in a significant increase in the production of activated protein C with a reduction in islet-mediated thrombogenicity. This report highlights the potential ...

Encapsulation and Surface Engineering of Pancreatic Islets: Advances and Challenges

Type 1 diabetes (T1D) is a chronic autoimmune disease representing a major health care problem worldwide (Tierney et al., 2002). T1D is caused by islet-reactive immune T cells that destroy insulin-producing pancreatic ┚-cells. Transplantation of insulin-producing pancreatic islets by their injection in vascularized organs has been recently recognized as a promising path to curing diabetes (Melo...

Toward Engineering a Novel Transplantation Site for Human Pancreatic Islets

Islet transplantation is a promising therapy for treatment of type 1 diabetes. A real breakthrough was reported when the Edmonton protocol was introduced in 2000. This protocol induced insulin independence in diabetic patients for 1 year (1). Although these clinical islet transplantations demonstrated the application of the technique, the long-term function of the islet grafts was not that succ...

Biomolecular Engineering

Biomolecular engineering is an emerging field of academic research and industrial practice having the goal of engineering value-added biomolecules and biomolecular processes for applications in medical, chemical, agricultural, and food industries. This new subject is very broad and highly interdisciplinary, including, but is not limited to, protein engineering, metabolic engineering, bioinforma...

Pancreatic Islets Engineered with SA-FasL